Journal: JACC: Basic to Translational Science

Article Title: JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in Cardiomyocytes

doi: 10.1016/j.jacbts.2026.101534

Figure Lengend Snippet: Ca 2+ Handling in Adult Mouse Cardiomyocytes (A to C) Adult mouse cardiomyocytes were infected with Ad-JP2 or Ad-HA and exposed to palmitate or oleate. RyR2 Ca 2+ leak (F/F0), caffeine-induced peak (F/F0), and Tau-SERCA were measured. (D to F) Adult mouse cardiomyocytes were infected with Ad-JCN or Ad-HA and exposed to palmitate or oleate. RyR2 Ca 2+ leak (F/F0), Caffeine-induced peak (F/F0), and Tau-SERCA were detected. (G to I) Adult mouse cardiomyocytes were infected with Ad-shJP2 or Ad-HA. RyR2 Ca 2+ leak (F/F0), caffeine-induced peak (F/F0), and Tau-SERCA were detected. (J to L) Adult mouse cardiomyocytes were infected with Ad-AsJCN or Ad-HA. RyR2 Ca 2+ leak (F/F0), caffeine-induced peak (F/F0), and Tau-SERCA were determined. Data are mean ± SD, n = 9-11 different adult mouse cardiomyocytes in each group. Two-way analysis of variance followed by Newman-Keuls post hoc test was performed for multiple pairwise comparisons. Student's t -test was used for comparison between 2 groups. ∗ P < 0.05 vs Ad-HA or oleate + Ad-HA, and † P < 0.05 vs palmitate + Ad-HA. ∗ and †: P < 0.05; ∗∗ and ††: P < 0.01; ∗∗∗ and †††: P < 0.001. Abbreviations as in .

Article Snippet: Then, sarcoplasmic reticulum (SR) Ca 2+ leak via RyRs was estimated in 0 Ca 2+ /0 Na + Tyrode's solution containing tetracaine (1 mmol/L, MedChemExpress, HY-A0079).

Techniques: Infection, Comparison

Journal: JACC: Basic to Translational Science

Article Title: JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in Cardiomyocytes

doi: 10.1016/j.jacbts.2026.101534

Figure Lengend Snippet: Schematic Mechanism In normal conditions, JP2 binds to JCN and blocks the MURF1-JCN interaction, thereby preventing MURF1-mediated ubiquitination and proteasome-dependent degradation of JCN. However, the protein level of JP2 is reduced under stress, enabling MURF1 to bind to JCN and promoting MURF1-mediated ubiquitination and degradation of JCN. JCN reduction triggers the excessive opening and Ca 2+ release from RyR2, leading to cardiomyocyte injury. In contrast, overexpression of JP2 or JCN protects intracellular Ca 2+ homeostasis and prevents cell death under stress.

Article Snippet: Then, sarcoplasmic reticulum (SR) Ca 2+ leak via RyRs was estimated in 0 Ca 2+ /0 Na + Tyrode's solution containing tetracaine (1 mmol/L, MedChemExpress, HY-A0079).

Techniques: Ubiquitin Proteomics, Over Expression

Journal: bioRxiv

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

doi: 10.64898/2026.03.30.715210

Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test

Journal: bioRxiv

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

doi: 10.64898/2026.03.30.715210

Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test